AN IMPROVED STAINING METHOD FOR ELECTRON MICROSCOPY
نویسندگان
چکیده
منابع مشابه
An Improved Staining Method for Electron Microscopy
A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...
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Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...
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Phase contrast, ultraviolet microscopy, and freeze-etching were used to determine the amount of exocellular polymer surrounding unfixed cells of four genera of bacteria: Azotobacter vinelandii, Zoogloea ramigera, Leuconostoc mesenteroides, and an acid-tolerant, floc-forming Bacillus species. Thin-sectional electron microscopy was employed to measure the effectiveness of a modified ruthenium red...
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An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chro...
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It has been the experience of this laboratory that the lead hydroxide stain of Watson (1) gives excellent results in increasing contrast in thin sections of tissue, but that the stain is inconvenient to use because on contact with air it forms an insoluble precipitate which contaminates both the stored solution and the sections. 1 Contamination introduced in transferring the solution from the s...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1964
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.22.3.697